I was teaching advanced Hebrew, and I had decided to take the class through the book of Judges. As we read along, I noticed something odd about Judges 3:10:
The Spirit of Yahweh came upon Caleb's younger brother...
In English, this passage from Judges doesn't appear startling, but in Hebrew something strange leapt out at me: "came upon" was a third person FEMININE verb, indicating it's subject "Spirit" was being understood as a feminine noun. Hebrew is not like Aramaic in its use of the word "spirit". While the word is exclusively feminine in Aramaic, in Hebrew it is sometimes masculine. Therefore, the question that came to mind was why had the author of Judges chosen here to make the Spirit of Yahweh feminine, when he could just as easily have made it masculine? Oh well.
I just shrugged my shoulders and went on, not overly concerned. Occasionally, I thought, one finds something inexplicable in the Bible: no big deal. But then came Judges 6:34. Again, "Spirit of Yahweh" was feminine.
At this point I decided to consult the concordance. Much to my surprise, every occurrence of "Spirit of Yahweh" in Judges is feminine. As I pondered that, I recalled Genesis 1:2, the first occurrence of "Spirit of God" in the Bible, and realized to my shock that it too is feminine.
Back to the concordance. Out of 84 OT uses of the word "spirit", in contexts traditionally assumed to be references to the Holy Spirit, 75 times it is either explicitly feminine or indeterminable (due to lack of a verb or adjective). Only nine times can "spirit" be construed as masculine, and in those cases it is unclear that it is a reference to God's Holy Spirit anyway. (Please see Appendix 3 for a complete list and detailed discussion of the usages.)
The New Testament references to the Holy Spirit are not helpful for conclusively deciding on the gender of the Holy Spirit, since "spirit" in Greek is neuter, and so is referred to as "it" by the New Testament writers.
The conclusion of all this is that our traditional assumption of a masculine Spirit is questionable; in fact, the evidence seems overwhelming that the Spirit should be viewed as "She", which does seem to make sense, since the other two members of the Godhead are labeled "Father" and "Son".
http://www.theology.edu/journal/volume3/spirit.htm